For industrial structure upgrading and modern service industry developing, China will need a large number of high-quality talents to contribute to new industrialization. China's higher education is important for cultivating high-quality, multi-skilled and well-trained talents. “What Knowledge is of most Worth?” that raised by Herbert Spencer is one of the most typical questions for education. After investigation, this study researches 2 questions based on China’s huge demand of well-trained, high-skilled, and application-oriented talents under current economic development: 1) “What Knowledge is of most Worth?” Practical Scientific Knowledge. 2) The guidance on the education practice. The essay demonstrates the challenges and provides constructive suggestions including reform education curriculum, strengthen faculty and enhance university-enterprise cooperation which is multi-win for undergraduates, universities and enterprises. Based on the university-enterprise cooperation practice with company M, the experiences are summarized and the research results can be popularized. This thesis will analyze Herbert Spencer’s “What Knowledge is of Most Worth?”, and provide the helpful advices on practical scientific knowledge education based on Herbert Spencer’s “What Knowledge is of Most Worth?”