Under the background of education modernization, construction of bilingual teaching courses in colleges and universities is directly driven by project of undergraduate teaching quality and teaching reform launched by the Ministry of Education, and has been expanding rapidly. This paper, takes the course of “introduction to e-commerce” as an object, measures the bilingual teaching effect from the four aspects, namely teachers’ professional capacity, teachers’ foreign language capacity, students’ learning motivation and suitability of teaching materials. The results of the study show that, students learning motivation, teachers’ professional capacity and teachers’ foreign language capacity significantly impact the bilingual teaching effect of “introduction to e-commerce”. Therefore, colleges and universities should actively guide students to interest in learning, stimulate students’ learning motivation, improve teachers’ foreign language level and enhance their professional quality, so as to improve the bilingual teaching effect of the course.